Ethanolic Extract of Moringa’s leaves (Moringa oleifera) Induce Senescence on Adenocarcinomic Alveolar Basal Epithelial Cells (A549 Cell-lines)

Abstract

Moringa (Moringa oleifera) is a plant that widely used as traditional medicines. The use of Moringa as herbal medicine until now is widely studied due to its high antioxidant activity and its role as an anticancer in vitro. This study aims to determine the effect of the ethanolic extract of Moringa's leaf on the viability and senescence of lung cancer cells (A549 cell-lines). Moringa leaves were extracted with 70% ethanol solvent and tested for cell viability by MTS test. Senescence detection test were conducted by Senescence Associated-β-Galactosidase (SA-β-Gal) staining. The result of the viability test showed that the ethanolic extract of Moringa leaves was able to reduce the viability of cancer cells by 1000 µg / ml of IC50 value. The senescence detection test results used SA-β-Gal showed that the ethanolic extract of Moringa leaves was able to induce senescence up to 25% in cancer cells. The same test also performed on normal cells (MCF-12A cell-lines). The result of the assay that conducted on normal cells showed that the ethanolic extract of Moringa leaves conduct greater toxicity in cancer cells, and did not significantly induce senescence in normal cells. These results indicate the potential of Moringa as a cancer therapy agent and could be used as a basis for further research on the potential for Moringa as an anticancer.